LeHavreV1, Main, Exploration, bibRecord, 000459

Sexual dimorphism in Grp78 and Hsp90A heat shock protein expression in the estuarine copepod Eurytemora affinis

Identifieur interne : 000459 ( Main/Exploration ); précédent : 000458; suivant : 000460

Sexual dimorphism in Grp78 and Hsp90A heat shock protein expression in the estuarine copepod Eurytemora affinis

Auteurs : Celine Boulangé-Lecomte [France] ; Joëlle Forget-Leray [France] ; Benoit Xuereb [France]

Source :

Cell Stress & Chaperones [ 1355-8145 ] ; 2013.

RBID : PMC:4041948

English descriptors

KwdEn :
- Animals, Arthropod Proteins (genetics), Copepoda (genetics), Copepoda (physiology), Female, Gene Expression Regulation, HSP90 Heat-Shock Proteins (genetics), Heat-Shock Proteins (genetics), Male, Reproduction, Sex Characteristics.
MESH :
- chemical , genetics : Arthropod Proteins, HSP90 Heat-Shock Proteins, Heat-Shock Proteins.
- genetics : Copepoda.
- physiology : Copepoda.
- Animals, Female, Gene Expression Regulation, Male, Reproduction, Sex Characteristics.

Abstract

Aquatic organisms are constantly exposed to both natural and anthropogenic stressors. Under stress conditions, they elicit a cellular stress response, involving heat shock proteins (HSPs). HSPs are essential to protect proteins against aggregation and to help in the folding of native proteins or refolding of damaged ones. Because of their conservation among taxons and their inducibility after environmental/chemical stress, HSPs are commonly used as ecological and ecotoxicological biomarkers. However, the appropriate use of such molecular tools requires the investigation of the influence of biotic factors on their basal levels. As a first step in biomarker characterization, the present study aims to evaluate the impact of the reproductive cycle on the expression of the two major HSPs, Grp78 and Hsp90A in the estuarine copepod Eurytemora affinis. The constitutive expression of both genes in males was weak when compared to female levels suggesting gender-specific stress tolerance. Transcript levels gradually increased during oogenesis and maximal levels were recorded in ovigerous females. The present data support the view that the reproductive condition of individuals has to be considered as a confounding factor in stress evaluation by HSP quantification.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4041948

DOI: 10.1007/s12192-013-0482-3
PubMed: 24337963
PubMed Central: 4041948

Affiliations:

Le document en format XML

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<author><name sortKey="Boulange Lecomte, Celine" sort="Boulange Lecomte, Celine" uniqKey="Boulange Lecomte C" first="Celine" last="Boulangé-Lecomte">Celine Boulangé-Lecomte</name>
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<front><div type="abstract" xml:lang="en"><p>Aquatic organisms are constantly exposed to both natural and anthropogenic stressors. Under stress conditions, they elicit a cellular stress response, involving heat shock proteins (HSPs). HSPs are essential to protect proteins against aggregation and to help in the folding of native proteins or refolding of damaged ones. Because of their conservation among taxons and their inducibility after environmental/chemical stress, HSPs are commonly used as ecological and ecotoxicological biomarkers. However, the appropriate use of such molecular tools requires the investigation of the influence of biotic factors on their basal levels. As a first step in biomarker characterization, the present study aims to evaluate the impact of the reproductive cycle on the expression of the two major HSPs, <italic>Grp78</italic>
 and <italic>Hsp90A</italic>
 in the estuarine copepod <italic>Eurytemora affinis</italic>
. The constitutive expression of both genes in males was weak when compared to female levels suggesting gender-specific stress tolerance. Transcript levels gradually increased during oogenesis and maximal levels were recorded in ovigerous females. The present data support the view that the reproductive condition of individuals has to be considered as a confounding factor in stress evaluation by HSP quantification.</p>
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Sexual dimorphism in Grp78 and Hsp90A heat shock protein expression in the estuarine copepod Eurytemora affinis

Sexual dimorphism in Grp78 and Hsp90A heat shock protein expression in the estuarine copepod Eurytemora affinis

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

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